The Pengaruh Ekstrak Bahan Organik Dan Iaa Terhadap Kultur In Vitro Anggrek (Phalaenopsis amabilis)

Abstract

Increasing the quality and quantity of orchid production in
vitro can be done by adding organic matter and growth regulators
(IAA). The purpose of this study was to determine the effect of
adding organic matter and IAA concentrations on orchid growth in
vitro. This research was conducted in the Kampoeng Anggrek tissue
culture laboratory, Sempu Village, Kediri Regency from November
2021 to February 2022. The design used was Completely
Randomized Design (CRD) with two factors and repeated 4 times.
The first factor was the addition of organic matter (A) consisting of
three levels, namely without organic matter, adding 150 g/L of
sweet potato extract and adding 150 g/L of Ambon banana extract.
The second factor was the concentration of IAA (B) consisting of
three levels, namely 0 ppm, 0.25 ppm and 0.5 ppm.
The results showed that the addition of 150 g/L sweet
potato and 0.25 ppm IAA increased root length. The addition of 150
g/L sweet potato and 0.5 ppm IAA increased the number of roots. In
the treatment without the addition of organic matter and 0.25 ppm
IAA can increase the length of plantlets. On a single factor the
addition of organic matter showed that the treatment with the
addition of 150 g/L sweet potato had the longest average root
length. The IAA single factor of 0.25 ppm shows the longest average
root length